SmartVac Project

Publicatii:

1) N-Glycosylation and N-Glycan Processing in HBV Biology and Pathogenesis. (2020) Dobrica MO, Lazar C, Branza-Nichita N. Cells. 9(6):E1404. Published 2020 Jun 4. doi:10.3390/cells9061404, IF 5,6

2) A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems. (2020) Codruta C. Popescu, Marius C. Stoian, Lia-Maria Cucos, Anca G. Coman, Antonio Radoi, Anca Paun, Niculina D. Hadade, Arnaud Gautier, Costin-Ioan Popescu and Mihaela Matache. RSC Adv., 10, 23931, IF 4,03

3) Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells. (2020) Mihaela-Olivia Dobrica, Catalin Lazar and Norica Branza-Nichita. Vaccine Delivery Technology: Methods and Protocols, Methods in Molecular Biology, Blaine Pfeifer and Andrew Hill (eds.), vol. 2183, https://doi.org/10.1007/978-1-0716-0795-4_7, © Springer Science+Business Media, LLC, part of Springer Nature. Book chapter.

4) Challenges and Prospects of Plant-Derived Oral Vaccines against Hepatitis B and C Viruses. (2021) Pantazica AM, Cucos LM, Stavaru C, Clarke JL, Branza-Nichita N. Plants (Basel), 10, 2037.https://doi.org/10.3390/plants10102037, IF 4,6

5) Hepatitis C virus E2 envelope glycoprotein produced in Nicotiana benthamiana triggers humoral response with virus-neutralizing activity in vaccinated mice. (2021) Dobrica MO, van Eerde A, Tucureanu C, Onu A, Paruch L, Caras I, Vlase E, Steen H, Haugslien S, Alonzi D, Zitzmann N, Bock R, Dubuisson J, Popescu CI, Stavaru C, Liu-Clarke J, Branza-Nichita N. Plant Biotechnology Journal, Oct;19(10):2027-2039, IF 9,8

6) Efficient cellular and humoral immune response and production of virus-neutralizing antibodies by the Hepatitis B Virus S/preS116-42 antigen (2022) Pantazica A-M, Dobrica MO, Lazar C, Scurtu C, Tucureanu C, Caras I, Ionescu I, Costcahe A, Onu A, Liu Clarke J, Stavaru C, Nichita N. Frontiers in Immunology, Jul 22;13:941243, IF 8.78

7) The "humanized" N-glycosylation pathway in CRISPR/Cas9-edited Nicotiana benthamiana significantly enhances the immunogenicity of a S/preS1 Hepatitis B Virus antigen and the virus-neutralizing antibody response in vaccinated mice (2023) Ana-Maria Pantazica, André van Eerde, Mihaela-Olivia Dobrica, Iuliana Caras, Irina Ionescu, Adriana Costache, Catalin Tucureanu, Hege Steen, Catalin Lazar, Inger Heldal, Sissel Haugslien, Adrian Onu, Crina Stavaru, Norica Branza-Nichita, Jihong Liu Clarke. Plant Biotechnology Journal, doi: 10.1111/pbi.14028. Online ahead of print, IF 13.26

Sustinere teza de doctorat:

Ana-Maria Mădălina Pantazică, (August 28, 2023) "Producerea si caracterizarea de particule subvirale chimerice pentru dezvoltarea unui nou vaccin fata de virusul hepatitei B" (rezumatul tezei de doctorat).

Conferinte:

1) A 49a Conferinta a Societatii Romane de Imunologie si a 4a Conferinta a Asociatiei Romane de Immuno-Dermatologie, 30.09-3.10.2020, Videoconferinta:

AM Pantazică*, OM Dobrică*, C Scurtu, N Nichita. "Production and characterization of novel HBV chimeric antigens". *Authors with equal contribution to this work.

LM Cucoș, TA Șulea, L.Spiridon, N Nichita, CI Popescu. “HCV Envelope Protein E2 Antigen Design and Characterization for Vaccine Development”

3) Simpozionul Anual al INCDMM “Cantacuzino”. 25.11.2021

Nichita N, “Production of novel vaccine antigens derived from the Human Hepatitis B Virus” (Key lecture)

4) Simpozionul Anual al "Protein Society" (USA), July 7-14, 2021

Pantazică A-M, Dobrică MO, Scurtu C, Nichita N, “Production and Characterization of Novel HBV Chimeric Antigens”, (poster presentation)

Cucos L-M, Sulea T, Caras I, Spiridon L, Stavaru C, Nichita N, Popescu CI, “Hepatitis C Virus envelope protein E2 based antigen design and characterization for vaccine development”, (poster presentation)

5) Conferinta Internationala Anuala a Societatii Romane de Biochimie si Biologie Moleculara, Iași, 26-27 Septembrie, 2019

MO Dobrica, L. Paruch, D. Alonzi, N. Zitzmann, CI Popescu, J. Dubuison, J. Liu-Clarke, N. Nichita, “Generation and characterization of HCV-E2 antigen in Nicotiana Benthamiana leaves for vaccine development”, (poster presentation)

6) 2022 International HBV Meeting, 18 – 22 September 2022, Paris, France.

Ana-Maria Pantazica, Mihaela-Olivia Dobrica, Catalin Lazar, Cristina Scurtu, Catalin Tucureanu, Iuliana Caras, Irina Ionescu, Adriana Costache, Adrian Onu, Jihong Liu- Clarke, Crina Stavaru, Norica Nichita. “Novel Hepatitis B S/preS1 chimeric antigen induces efficient cellular and humoral immune response and production of virus-neutralizing antibodies”, (prezentare poster)

7) FEBS-IUBMB-ENABLE 2022, 16 - 18 November 2022, Seville, Spain

Ana-Maria Pantazica, Mihaela-Olivia Dobrica, Catalin Lazar, Cristina Scurtu, Catalin Tucureanu, Iuliana Caras, Irina Ionescu, Adriana Costache, Adrian Onu, Jihong Liu- Clarke, Crina Stavaru, Norica Branza-Nichita. "Novel chimeric Hepatitis B S/preS1 antigen induces efficient cellular and humoral immune response and production of virus-neutralizing antibodies" (scurta prezentare orala)

Cucos Lia-Maria, Teodor Asvadur Sulea, Laurentiu Spiridon, Norica Nichita, Costin-Ioan Popescu. "Hepatitis C Virus Envelope Protein E2 Antigen Design and Characterization for Vaccine Development", (prezentare poster)

8) Conferinta Anuala a Studentilor Doctoranzi ai Institutului de Biochimie, 12 Decembrie, 2022

Ana-Maria Pantazica, Mihaela-Olivia Dobrica, Catalin Lazar, Cristina Scurtu, Catalin Tucureanu, Iuliana Caras, Irina Ionescu, Adriana Costache, Adrian Onu, Jihong Liu- Clarke, Crina Stavaru, Norica Nichita “Production and characterization of chimeric subviral particles for the development of a novel HBV vaccine”, (prezentare orala)

Cucos Lia-Maria, Teodor Asvadur Sulea, Laurentiu Spiridon, Norica Nichita, Costin-Ioan Popescu.“Production and characterization of HCV antigens based on the envelope protein E2, for vaccine development”, (prezentare orala)

9) Conferinta anuala a RSBMB, 13-15 Septembrie 2023, Cluj-Napoca

Nichita Norica Beatrice "Production of chimeric subviral particles with enhanced immunogenicity for the development of next- generation HBV vaccines", (prezentare orala).

Pantazica Ana-Maria, Eerde Andre, Dobrica Olivia, Caras Iuliana, Ionescu Irina, Adriana Costache, Tucureanu Catalin, Steen Hege, Lazar Catalin, Heldal Inger, Haugslien Sissel, Onu Adrian, Stavaru Georgeta-Crina, Liu Clarke Jihong, Nichita Norica Beatrice. ""Humanization” of the N-glycosylation pathway in Nicotiana benthamiana via CRISPR/Cas9 technology significantly improves the immunogenicity of HBV antigens and the virus- neutralizing antibody response in vaccinated mice", (prezentare orala).

Cucos Lia-Maria, Sulea Teodor Asvadur, Laurentiu Spiridon, Caras Iuliana, Ionescu Irina, Adriana Costache, Stavaru Georgeta-Crina, Nichita Norica Beatrice, Popescu Costin-Ioan. "Hepatitis C Virus Envelope Protein E2 Antigen Design and Characterization for Vaccine Development", (prezentare orala).

Dobrica Olivia "Plant-produced Hepatitis C Virus E2-derived glycoproteins for vaccine development", (prezentare orala).